An immunofluorescent method for identifying individual IFN-γ-producing lymphocytes

Tamás Laskay, Ulf Andersson*, Jan Andersson, Rolf Kiessling, Marc DeLey

*Korrespondierende/r Autor/-in für diese Arbeit
21 Zitate (Scopus)

Abstract

An immunofluorescent staining method was developed for detecting human IFN-γ-producing cells in single cell suspensions. Mononuclear leukocytes, stimulated in vitro to produce IFN-γ, were fixed and made permeable. The cytoplasmic presence of IFN-γ was visualized by indirect immunofluorescence using IFN-γ-specific mouse monoclonal antibodies. The staining was found to be specific for IFN-γ and allowed the detection of newly synthesized rather than internalized IFN-γ molecules. The cytoplasmic fluorescence appeared locally in a polar, juxtanuclear position, which overlapped the Golgi apparatus, probably reflecting the glycosylation site of the newly formed IFN-γ molecules. Two-colour staining experiments showed that the method is useful not only for the detection and enumeration but also for the phenotypic characterization of IFN-γ-producing cells.

OriginalspracheEnglisch
ZeitschriftJournal of Immunological Methods
Jahrgang95
Ausgabenummer1
Seiten (von - bis)1-7
Seitenumfang7
ISSN0022-1759
DOIs
PublikationsstatusVeröffentlicht - 04.12.1986

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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