Abstract
An immunofluorescent staining method was developed for detecting human IFN-γ-producing cells in single cell suspensions. Mononuclear leukocytes, stimulated in vitro to produce IFN-γ, were fixed and made permeable. The cytoplasmic presence of IFN-γ was visualized by indirect immunofluorescence using IFN-γ-specific mouse monoclonal antibodies. The staining was found to be specific for IFN-γ and allowed the detection of newly synthesized rather than internalized IFN-γ molecules. The cytoplasmic fluorescence appeared locally in a polar, juxtanuclear position, which overlapped the Golgi apparatus, probably reflecting the glycosylation site of the newly formed IFN-γ molecules. Two-colour staining experiments showed that the method is useful not only for the detection and enumeration but also for the phenotypic characterization of IFN-γ-producing cells.
Originalsprache | Englisch |
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Zeitschrift | Journal of Immunological Methods |
Jahrgang | 95 |
Ausgabenummer | 1 |
Seiten (von - bis) | 1-7 |
Seitenumfang | 7 |
ISSN | 0022-1759 |
DOIs | |
Publikationsstatus | Veröffentlicht - 04.12.1986 |
Strategische Forschungsbereiche und Zentren
- Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)