An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä; Hanna Ulrich; Susi Dürr; Dominik Soll; Lynette Henkel; Corinne Angerpointner; Julia Ritter; Sabine Przibilla; Herbert Stadler; Manuel Effenberger; Dirk H. Busch; Matthias Schiemann

doi:10.1002/cyto.a.23964

An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä, Hanna Ulrich, Susi Dürr, Dominik Soll, Lynette Henkel, Corinne Angerpointner, Julia Ritter, Sabine Przibilla, Herbert Stadler, Manuel Effenberger, Dirk H. Busch, Matthias Schiemann^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Systemische Entzündungsforschung

38 Zitate (Scopus)

Abstract

Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.

Originalsprache	Englisch
Zeitschrift	Cytometry Part A
Jahrgang	97
Ausgabenummer	2
Seiten (von - bis)	171-183
Seitenumfang	13
ISSN	1552-4922
DOIs	https://doi.org/10.1002/cyto.a.23964
Publikationsstatus	Veröffentlicht - 01.02.2020

Fördermittel

This work was in part funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) ? ?Projektnummer 272983813? ? TRR 179. The authors thank Philipp Bruns (DKFZ, Heidelberg) and Lena Appel (MIH, M?nchen) for help with data processing and visualization of MicroArray data.

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Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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10.1002/cyto.a.23964

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title = "An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation",

abstract = "Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.",

author = "Immanuel Andr{\"a} and Hanna Ulrich and Susi D{\"u}rr and Dominik Soll and Lynette Henkel and Corinne Angerpointner and Julia Ritter and Sabine Przibilla and Herbert Stadler and Manuel Effenberger and Busch, \{Dirk H.\} and Matthias Schiemann",

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doi = "10.1002/cyto.a.23964",

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T1 - An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

AU - Andrä, Immanuel

AU - Ulrich, Hanna

AU - Dürr, Susi

AU - Soll, Dominik

AU - Henkel, Lynette

AU - Angerpointner, Corinne

AU - Ritter, Julia

AU - Przibilla, Sabine

AU - Stadler, Herbert

AU - Effenberger, Manuel

AU - Busch, Dirk H.

AU - Schiemann, Matthias

PY - 2020/2/1

Y1 - 2020/2/1

N2 - Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.

AB - Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.

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M3 - Journal articles

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An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Abstract

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