Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

Maja Živković; Aleksandar Raković; Aleksandra Stanković

doi:10.1016/j.clinbiochem.2006.01.005

Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

Maja Živković^*, Aleksandar Raković, Aleksandra Stanković

^*Korrespondierende/r Autor/-in für diese Arbeit

Klinik für Neurologie

5 Zitate (Scopus)

Abstract

Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.

Originalsprache	Englisch
Zeitschrift	Clinical Biochemistry
Jahrgang	39
Ausgabenummer	6
Seiten (von - bis)	630-632
Seitenumfang	3
ISSN	0009-9120
DOIs	https://doi.org/10.1016/j.clinbiochem.2006.01.005
Publikationsstatus	Veröffentlicht - 01.06.2006

Fördermittel

This work was supported by a Serbian Government Research Grant (5023). We greatly appreciate Dr. Dragan Alavantić for helpful suggestions during preparation of this paper.

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10.1016/j.clinbiochem.2006.01.005

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title = "Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE",

abstract = "Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.",

author = "Maja {\v Z}ivkovi{\'c} and Aleksandar Rakovi{\'c} and Aleksandra Stankovi{\'c}",

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doi = "10.1016/j.clinbiochem.2006.01.005",

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AU - Živković, Maja

AU - Raković, Aleksandar

AU - Stanković, Aleksandra

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.

AB - Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.

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U2 - 10.1016/j.clinbiochem.2006.01.005

DO - 10.1016/j.clinbiochem.2006.01.005

M3 - Journal articles

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SP - 630

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JF - Clinical Biochemistry

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ER -

Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

Abstract

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