A recombinant HAV expressing a neutralization epitope of HEV induces immune response against HAV and HEV in mice

Kui Xiang; Yuri Kusov; Guan Ying; Wang Yan; Yi Shan; Wu Jinyuan; Yin Na; Zhou Yan; Li Hongjun; Sun Maosheng

doi:10.3390/v9090260

A recombinant HAV expressing a neutralization epitope of HEV induces immune response against HAV and HEV in mice

Kui Xiang, Yuri Kusov, Guan Ying, Wang Yan, Yi Shan, Wu Jinyuan, Yin Na, Zhou Yan, Li Hongjun^*, Sun Maosheng

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biochemie

4 Zitate (Scopus)

Abstract

Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.

Originalsprache	Englisch
Aufsatznummer	260
Zeitschrift	Viruses
Jahrgang	9
Ausgabenummer	9
DOIs	https://doi.org/10.3390/v9090260
Publikationsstatus	Veröffentlicht - 15.09.2017

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Conflicts of Interest: No conflicting financial interests exist. This work was supported by grant (2016-I2M-1-019) from the CAMS Initiative for Innovative Medicine and Science and Technology Plan Project of Yunnan Province (2014BC008; 2016FB034). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study.

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title = "A recombinant HAV expressing a neutralization epitope of HEV induces immune response against HAV and HEV in mice",

abstract = "Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.",

author = "Kui Xiang and Yuri Kusov and Guan Ying and Wang Yan and Yi Shan and Wu Jinyuan and Yin Na and Zhou Yan and Li Hongjun and Sun Maosheng",

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T1 - A recombinant HAV expressing a neutralization epitope of HEV induces immune response against HAV and HEV in mice

AU - Xiang, Kui

AU - Kusov, Yuri

AU - Ying, Guan

AU - Yan, Wang

AU - Shan, Yi

AU - Jinyuan, Wu

AU - Na, Yin

AU - Yan, Zhou

AU - Hongjun, Li

AU - Maosheng, Sun

PY - 2017/9/15

Y1 - 2017/9/15

N2 - Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.

AB - Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.

UR - https://www.scopus.com/pages/publications/85029612668

U2 - 10.3390/v9090260

DO - 10.3390/v9090260

M3 - Journal articles

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SN - 1999-4915

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JO - Viruses

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A recombinant HAV expressing a neutralization epitope of HEV induces immune response against HAV and HEV in mice

Abstract

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