A common core for binding single-stranded DNA: Structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria

Gordon Webster; Jochen Genschel; Ute Curth; Claus Urbanke; Chul Hee Kang; Rolf Hilgenfeld

doi:10.1016/S0014-5793(97)00747-3

A common core for binding single-stranded DNA: Structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria

Gordon Webster^*, Jochen Genschel, Ute Curth, Claus Urbanke, Chul Hee Kang, Rolf Hilgenfeld

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biochemie

63 Zitate (Scopus)

Abstract

The crystal structure of the DNA-binding domain of E. coli SSB (EcoSSB) has been determined to a resolution of 2.5 A. This is the first reported structure of a prokaryotic SSB. The structure of the DNA-binding domain of the E. coli protein is compared to that of the human mitochondrial SSB (HsmtSSB). In spite of the relatively low sequence identity between them, the two proteins display a high degree of structural similarity. EcoSSB crystallises with two dimers in the asymmetric unit, unlike HsmtSSB which contains only a dimer. This is probably a consequence: of the different polypeptide chain lengths in the EcoSSB heterotetramer. Crucial differences in the dimer-dimer interface of EcoSSB may account for the inability of EcoSSB and HsmtSSB to form cross-species heterotetramers, in contrast to many bacterial SSBs.

Originalsprache	Englisch
Zeitschrift	FEBS Letters
Jahrgang	411
Ausgabenummer	2-3
Seiten (von - bis)	313-316
Seitenumfang	4
ISSN	0014-5793
DOIs	https://doi.org/10.1016/S0014-5793(97)00747-3
Publikationsstatus	Veröffentlicht - 14.07.1997

Fördermittel

We thank F. Pasutto and J.R. Mesters for improving the EcoSSB crystallisation procedure and D. Geller, U. Gröbner, J. Hoven and L. Litz for technical support. The expert advice of A. Savoia and his coworkers during data collection at Sincrotrone Trieste is gratefully acknowledged. Work at Hannover was supported by the Deutsche Forschungsgemeinschaft.

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10.1016/S0014-5793(97)00747-3

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title = "A common core for binding single-stranded DNA: Structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria",

abstract = "The crystal structure of the DNA-binding domain of E. coli SSB (EcoSSB) has been determined to a resolution of 2.5 A. This is the first reported structure of a prokaryotic SSB. The structure of the DNA-binding domain of the E. coli protein is compared to that of the human mitochondrial SSB (HsmtSSB). In spite of the relatively low sequence identity between them, the two proteins display a high degree of structural similarity. EcoSSB crystallises with two dimers in the asymmetric unit, unlike HsmtSSB which contains only a dimer. This is probably a consequence: of the different polypeptide chain lengths in the EcoSSB heterotetramer. Crucial differences in the dimer-dimer interface of EcoSSB may account for the inability of EcoSSB and HsmtSSB to form cross-species heterotetramers, in contrast to many bacterial SSBs.",

author = "Gordon Webster and Jochen Genschel and Ute Curth and Claus Urbanke and Kang, \{Chul Hee\} and Rolf Hilgenfeld",

note = "Funding Information: We thank F. Pasutto and J.R. Mesters for improving the EcoSSB crystallisation procedure and D. Geller, U. Gr{\"o}bner, J. Hoven and L. Litz for technical support. The expert advice of A. Savoia and his coworkers during data collection at Sincrotrone Trieste is gratefully acknowledged. Work at Hannover was supported by the Deutsche Forschungsgemeinschaft. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1997",

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doi = "10.1016/S0014-5793(97)00747-3",

language = "English",

volume = "411",

pages = "313--316",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "2-3",

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A common core for binding single-stranded DNA: Structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria. / Webster, Gordon; Genschel, Jochen; Curth, Ute et al.
in: FEBS Letters, Jahrgang 411, Nr. 2-3, 14.07.1997, S. 313-316.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - A common core for binding single-stranded DNA: Structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria

AU - Webster, Gordon

AU - Genschel, Jochen

AU - Curth, Ute

AU - Urbanke, Claus

AU - Kang, Chul Hee

AU - Hilgenfeld, Rolf

N1 - Funding Information: We thank F. Pasutto and J.R. Mesters for improving the EcoSSB crystallisation procedure and D. Geller, U. Gröbner, J. Hoven and L. Litz for technical support. The expert advice of A. Savoia and his coworkers during data collection at Sincrotrone Trieste is gratefully acknowledged. Work at Hannover was supported by the Deutsche Forschungsgemeinschaft. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1997/7/14

Y1 - 1997/7/14

N2 - The crystal structure of the DNA-binding domain of E. coli SSB (EcoSSB) has been determined to a resolution of 2.5 A. This is the first reported structure of a prokaryotic SSB. The structure of the DNA-binding domain of the E. coli protein is compared to that of the human mitochondrial SSB (HsmtSSB). In spite of the relatively low sequence identity between them, the two proteins display a high degree of structural similarity. EcoSSB crystallises with two dimers in the asymmetric unit, unlike HsmtSSB which contains only a dimer. This is probably a consequence: of the different polypeptide chain lengths in the EcoSSB heterotetramer. Crucial differences in the dimer-dimer interface of EcoSSB may account for the inability of EcoSSB and HsmtSSB to form cross-species heterotetramers, in contrast to many bacterial SSBs.

AB - The crystal structure of the DNA-binding domain of E. coli SSB (EcoSSB) has been determined to a resolution of 2.5 A. This is the first reported structure of a prokaryotic SSB. The structure of the DNA-binding domain of the E. coli protein is compared to that of the human mitochondrial SSB (HsmtSSB). In spite of the relatively low sequence identity between them, the two proteins display a high degree of structural similarity. EcoSSB crystallises with two dimers in the asymmetric unit, unlike HsmtSSB which contains only a dimer. This is probably a consequence: of the different polypeptide chain lengths in the EcoSSB heterotetramer. Crucial differences in the dimer-dimer interface of EcoSSB may account for the inability of EcoSSB and HsmtSSB to form cross-species heterotetramers, in contrast to many bacterial SSBs.

UR - https://www.scopus.com/pages/publications/0031567573

U2 - 10.1016/S0014-5793(97)00747-3

DO - 10.1016/S0014-5793(97)00747-3

M3 - Journal articles

C2 - 9271227

AN - SCOPUS:0031567573

SN - 0014-5793

VL - 411

SP - 313

EP - 316

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

A common core for binding single-stranded DNA: Structural comparison of the single-stranded DNA-binding proteins (SSB) from E. coli and human mitochondria

Abstract

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