A bioinformatic assay for pluripotency in human cells

Franz Josef Müller; Bernhard M. Schuldt; Roy Williams; Dylan Mason; Gulsah Altun; Eirini P. Papapetrou; Sandra Danner; Johanna E. Goldmann; Arne Herbst; Nils O. Schmidt; Josef B. Aldenhoff; Louise C. Laurent; Jeanne F. Loring

doi:10.1038/nmeth.1580

A bioinformatic assay for pluripotency in human cells

Franz Josef Müller^*, Bernhard M. Schuldt, Roy Williams, Dylan Mason, Gulsah Altun, Eirini P. Papapetrou, Sandra Danner, Johanna E. Goldmann, Arne Herbst, Nils O. Schmidt, Josef B. Aldenhoff, Louise C. Laurent, Jeanne F. Loring

^*Korrespondierende/r Autor/-in für diese Arbeit

230 Zitate (Scopus)

Abstract

Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.

Originalsprache	Englisch
Zeitschrift	Nature Methods
Jahrgang	8
Ausgabenummer	4
Seiten (von - bis)	315-317
Seitenumfang	3
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.1580
Publikationsstatus	Veröffentlicht - 01.04.2011
Extern publiziert	Ja

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abstract = "Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.",

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AU - Altun, Gulsah

AU - Papapetrou, Eirini P.

AU - Danner, Sandra

AU - Goldmann, Johanna E.

AU - Herbst, Arne

AU - Schmidt, Nils O.

AU - Aldenhoff, Josef B.

AU - Laurent, Louise C.

AU - Loring, Jeanne F.

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AB - Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.

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A bioinformatic assay for pluripotency in human cells

Abstract

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